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KMID : 1123920080220051315
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Korean Journal of Oriental Physiology and Pathology
2008 Volume.22 No. 5 p.1315 ~ p.1321
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Inhibitory Effect of Chan-Su on the Secretion of PGE2 and NO in LPS-stimulated BV2 Microglial Cells
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Kim Min-Hee
Kim Won-Ill
Hong Sang-Hoon
Lyu Ji-Hyo
Lyu Sun-Ae
Yoon Hwa-Jung
Ko Woo-Shin
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Abstract
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Chan-Su (Venenum bufonis) has long been for a variety of other purposes including treatment of inflammation in the folk medicine recipe. Since nitric oxide (NO) is one of the major inflammatory parameters, we first studied the effects of Chan-Su on NO production in lipopolysaccharide (LPS)-stimulated BV2 microglial cells, Chan-Su inhibited the secretion of NO in BV2 microglial cells, without affecting cell viability, The protein level of inducible nitric oxide synthase (iNOS) was decreased by Chan-Su, And Chan-Su also inhibited production of prostaglandin E2 (PGE2) and expression of cyclooxygenase (COX)-2. Proinflammatory cytokines, such as tumor necrosis factor , interleukin and IL-12, were inhibited by Chan-Su in a dose-dependent manner. And Chan-Su inhibited the degradation of , which was considered to be inhibitor of nuclear factor , one of a potential transcription factor for the expression of iNOS, COX-2 and proinflammatory cytokines. These results suggest that Chan-Su could exert its anti-inflammatory actions by suppressing the synthesis of NO through inhibition of degradation.
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KEYWORD
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Chan-Su(Venenum bufonis), nitric oxide, inducible nitric oxide synthase, cyclooxygenase-2, inhibitor kappa B
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